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Gene Structure Display Server (GSDS) is designed for the visualization of gene features, such as the composition and position of exons, introns, and conserved elements .etc. The input could be sequences, GenBank Accession Number (or GI), or features in BED/GTF/GFF3 formats. After inputting gene features, a high-quality image can be generated. Shape and color for features can be customized by users and further modifying functions on figures are provided. To facilitate evolutionary analysis, a phylogenetic tree can be uploaded and added on the figure. In addition, the source code for GSDS website is available for downloading. Compared with version 1.0, GSDS 2.0 has a newly designed user-interface and several new features as following: Support for multiple widely-used gene feature description formats including BED, GTF/GFF3 has been added. Besides exons and introns, more types of annotation features such as conserved-elements, binding sites and other functional elements can be displayed with customizable shapes/colors. The figure can be modified in feature display after its first generation, and sent to a built-in interactive editor for further refining. To facilitate evolutionary analysis, a phylogenetic tree can be uploaded and added to the side of the gene structure.